Improvement of transformation efficiency by strategic circumvention of restriction barriers in Streptomyces griseus.

Oxidation of Meloxicam by Streptomyces griseus

The aim of the present investigation was to biotransform the anti-inflammatory compound meloxicam by enzymes present in whole cells of five actinomycete cultures to produce novel bioactive derivatives. Among the actinomycetes screened, Streptomyces griseus NCIM 2622 was found to possess the enzyme system(s) that oxidize meloxicam into two metabolites whereas that present in S. griseus NCIM 2623...

Strain- and species-specific distribution of the streptomycin gene cluster and kan-related sequences in Streptomyces griseus.

The streptomycin (SM) gene cluster was investigated for its distribution in streptomycetes by Southern hybridization using nick-translated DNA probes, which were isolated from the SM-6-phosphotransferase (SPH) and amidinotransferase (ADT) regions of the SM gene cluster of Streptomyces griseus SS-1198. Bgl II-digested genomic DNAs from SM-producing strains of S. griseus yielded the same size fra...

جداسازی و تأیید مولکولی سریع استرپتومایسس های تولید کننده آنتی بیوتیک استرپتومایسین

Introduction: Streptomyces species are mycelial, aerobic gram-positive bacteria that are isolated from soil and produce a diverse range of antibiotics. Streptomyces griseus produces the antibiotic, streptomycin and forms spores even in a liquid culture. The gene cluster for the production of Streptomycin antibiotic contains strR gene that encodes StrR, a pathway-specific regulator. Then, this p...

Isolation and characterization of a phage active against streptomyces griseus

Barriers to the Implementation of the Strategic Plan from the Viewpoint of Managers of the Hospitals of Qom Province in 2019 (Iran)

Background and Objectives: Increasing complexity and changes in environmental conditions, policies, attitudes, systems, and structures, are factors influencing the goals of strategic planning, which can change programs. Programs without strategic thinking are not able to cope with such changes, thus they fail. Understanding of the factors affecting strategic planning, is crucial for the goals t...